Applying ‘MRM Spectrum Mode’ and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis

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In a regulated environment such as food safety there is a growing need to enhance the capability in routine monitoring programs by increasing the number of pesticides measured in a single analysis and at the same time delivering the highest confidence in compound identification to reduce false detect reporting. To this end, routine multiple-reaction monitoring (MRM) methods have been extended to monitor a higher number of fragment ion transitions to enhance specificity and reporting confidence. For each target pesticide, responses of multiple fragment ion transitions were assembled and displayed as spectra, which could be used in routine library searching and compound verification using reference library match scores.

Described in this article is an example of this ‘MRM Spectrum Mode’ being applied to quantify and identify, in spiked food matrix, 193 pesticides using 1,291 MRM transitions. The advantage of this technique is that library searchable product ion spectrum data is used in target compound identification without compromising sensitivity, accuracy and robustness in quantitative data reporting. As the method involves no “triggering”, it is robust and the data produced is consistent regardless of coexisting pesticide residues.

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