Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil Using ISOLUTE® EPH SPE Columns on Biotage® Extrahera™

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This application note describes the automated fractionation of extractable petroleum hydrocarbons (EPH) from soil samples into aliphatic and aromatic fractions using ISOLUTE^® EPH SPE columns in conjunction with the Biotage^® Extrahera^TM automation system. 24 samples can be efficiently fractionated in 42 minutes.

The principle is similar to the approach taken by the
Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimized. This allows an automation compatible
method of fractionation, which overcomes the
common problem of PAH breakthrough into the aliphatic fraction, in addition to reduced solvent volumes.

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