Determination of Amino Acid Composition of Cell Culture Media and Protein Hydrosylate Standard

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This study presents a method for analysing primary amino acids in cell culture media. Derivitization using an online injector program with OPA and FMOC decreases sample preparation time, and increases reproducibility over traditional offline methods. 

The effectiveness of this solution for routine analysis was confirmed using a system suitability test and retention time and area precision studies. The AdvanceBio AAA solution provides sensitive and high‑resolution separation of all amino acids in cell culture media. The limit of detection (LOD), limit of quantification (LOQ), and linearity for selected amino acids for qualitative assays are also reported.

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