Fast, Accurate LC-MS/MS Analysis of the C3 Epimer of 25-Hydroxyvitamin D on Raptor FluoroPhenyl Columns

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Vitamin D analysis has increased dramatically in clinical practice due to its association with multiple human diseases including cancer, rheumatoid arthritis, and diabetes. It also has an important role in calcium and phosphorous absorption in relation to bone strength, so there is growing concern about the prevalence of vitamin D deficiency worldwide. Accurate LC analysis of vitamin D epimers is critical for quantitative determination of the bioactive forms of vitamin D.

This application note shows how the Raptor FluoroPhenyl column is a good choice for vitamin D analysis as it provides alternate selectivity to a C18 column and completely separates the isobaric epimers and biomarkers in just 5 minutes. The columns improve LC analysis of vitamin D epimers, reliably providing accurate measurement of vitamin D content for disease and deficiency investigation and monitoring.

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