The focus of this technical note is N-glycosylation profiling of carbohydrates associated with therapeutic antibodies for faster, more cost-effective development of biologics. The goal is to demonstrate the advantages of the SCIEX Fast Glycan Labelling and Analysis technology for preparation and analysis of N-linked oligosaccharides.
The technical note demonstrates how fast glycan labelling and analysis technology reduced sample preparation time to roughly one hour with excellent yield and high reproducibility. Subsequent analysis took only 5 minutes and provided automated, immediate glycan identification.
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