Venoms are complex biological fluids that contain unique mixtures of peptides and proteins. Identifying and quantifying the composition of venoms is of increasing scientific interest. It is especially important to characterize the toxins responsible for the severe biological effects of the venom on humans. Better understanding of the clinical symptoms of envenoming would help with the development of effective therapies. Also, venoms are being investigated as potential sources of new compounds in drug development.
Absolute quantification of snake venom proteins is described. Using capillary LC to separate the proteins, followed by sulfur determination using isotope dilution ICP-QQQ, accurate concentrations were obtained without protein-specific standards.
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