This application note highlights the benefits of automated high-throughput trypsin digestion for highly reproducible peptide mapping of monoclonal antibodies using the Thermo Scientific™ SMART Digest™ Kit on the Thermo Scientific™ KingFisher™ Duo Prime purification system.
The study focused on reproducibility, protein sequence coverage, and identification of post-translational modifications (PTMs), including deamidation, oxidation, N-terminal pyroglutamination, C-terminal lysine loss, glycation, and glycosylation.
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