---
title: Analysis of Aflatoxins by UHPLC with MS/MS Detection Form
description: "This application note demonstrates the effective chromatographic separation of aflatoxins B1, B2, G1, G2\nand M1 by UHPLC-MS/MS, achieving the 0.05-ppb control limit for M1 without the need for derivatization."
image: https://appnote.sepscience.com/hubfs/Perkin%20Elmer/Food_Apps/PE-analysis-of-aflatoxins.png
---

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# Analysis of Aflatoxins by UHPLC with MS/MS Detection

![PE-analysis-of-aflatoxins](https://appnote.sepscience.com/hs-fs/hubfs/Perkin%20Elmer/Food_Apps/PE-analysis-of-aflatoxins.png?width=300&name=PE-analysis-of-aflatoxins.png)

## Download your copy...

Aflatoxins are some of the most carcinogenic mycotoxins known and include aflatoxins B1, B2, G1 and G2. They are produced by toxigenic strains of *Aspergillus flavus*, *Aspergillus nominus*, and *Aspergillus parasiticus* fungi after feed, crop or harvest exposure to moisture or warm temperatures.

One of the strictest regulatory levels for any aflatoxin has been set forth by the European Union  
(EU), having established a stringent control limit of 0.05 ppb for M1 in milk. This limit is  
currently significantly lower than the Food and Drug Administration’s (FDA’s) limit of 0.5 ppb.

For aflatoxin analysis, HPLC with fluorescence (FL) detection is commonly used along with online post-column derivatization, to enhance B1 and G1 sensitivity. However, chromatographers  
prefer to avoid any derivatization requirements. With this in mind, the work presented herein  
demonstrates the effective chromatographic separation of aflatoxins B1, B2, G1, G2  
and M1 by UHPLC-MS/MS, achieving the 0.05-ppb control limit for M1 without the need  
for derivatization.

To download your copy of this application note complete the form opposite.

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