Aflatoxins are some of the most carcinogenic mycotoxins known and include aflatoxins B1, B2, G1 and G2. They are produced by toxigenic strains of Aspergillus flavus, Aspergillus nominus, and Aspergillus parasiticus fungi after feed, crop or harvest exposure to moisture or warm temperatures.
One of the strictest regulatory levels for any aflatoxin has been set forth by the European Union
(EU), having established a stringent control limit of 0.05 ppb for M1 in milk. This limit is
currently significantly lower than the Food and Drug Administration’s (FDA’s) limit of 0.5 ppb.
For aflatoxin analysis, HPLC with fluorescence (FL) detection is commonly used along with online post-column derivatization, to enhance B1 and G1 sensitivity. However, chromatographers
prefer to avoid any derivatization requirements. With this in mind, the work presented herein
demonstrates the effective chromatographic separation of aflatoxins B1, B2, G1, G2
and M1 by UHPLC-MS/MS, achieving the 0.05-ppb control limit for M1 without the need
for derivatization.
To download your copy of this application note complete the form opposite.
Registrants for this application note are covered by the Eclipse Business Media Privacy Policy.
Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, info@sepscience.com
Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy or contact privacy@labxmediagroup.com
Copyright © 2025. All rights reserved