Analysis of Glyphosate, Glufosinate and Metabolites Using UCT’s Enviro-Clean® Glyphosate Push-Thru Purification Cartridge

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Glyphosate (N-(phosphonomethyl)glycine) is a broad-spectrum systemic herbicide and crop desiccant that is used to kill weeds and grasses that compete with crops. While glyphosate formulations have been approved by regulatory bodies worldwide, concerns about its effects on humans and the environment have grown as the global usage of glyphosate has increased.

UCT’s Enviro-Clean^® Glyphosate Push-Thru Purification Cartridge is shown to simplifiy the analysis of glyphosate and its metabolites by removing unwanted matrix components that can otherwise lead to significant ion-suppression. This application note outlines a suggested extraction procedure and an optional fluorenylmethyloxycarbonyl chloride (FMOC) derivatization step that allows for the analysis of glyphosate on a C18 HPLC column. However, the procedure can readily be modified to meet a laboratory’s specific sample volume and analysis requirements.

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