---
title: Comparison of Alternative Approaches to Trypsin Protein Digestion for Reproducible and Efficient Peptide Mapping Analysis of Monoclonal Antibodies Form
description: Comparison of peptide mapping data acquired using the Thermo Scientific™ SMART Digest™ kit and Trypsin kit, Magnetic Bulk Resin option, obtained by alternative digestion methods including an in-solution protocol and a rapid protein digestion method.
image: https://appnote.sepscience.com/hubfs/Featured_Applications/Oct_18/Thermo-monitoring-of-mammalian-cell-culture-media.png
---

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# Comparison of Alternative Approaches to Trypsin Protein Digestion for Reproducible and Efficient Peptide Mapping Analysis of Monoclonal Antibodies

![Thermo-monitoring-of-mammalian-cell-culture-media](https://appnote.sepscience.com/hs-fs/hubfs/Featured_Applications/Oct_18/Thermo-monitoring-of-mammalian-cell-culture-media.png?width=300&name=Thermo-monitoring-of-mammalian-cell-culture-media.png)

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Comparison of peptide mapping data acquired using the Thermo Scientific™ SMART Digest™ kit and Trypsin kit, Magnetic Bulk Resin option, obtained by alternative digestion methods including an in-solution protocol and a rapid protein digestion method.

The study focused on reproducibility, protein  
sequence coverage and identification of post-translational modifications (PTMs), including deamidation and oxidation.

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