Comparison of Alternative Approaches to Trypsin Protein Digestion for Reproducible and Efficient Peptide Mapping Analysis of Monoclonal Antibodies

Thermo-monitoring-of-mammalian-cell-culture-media

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Comparison of peptide mapping data acquired using the Thermo Scientific™ SMART Digest™ kit and Trypsin kit, Magnetic Bulk Resin option, obtained by alternative digestion methods including an in-solution protocol and a rapid protein digestion method.

The study focused on reproducibility, protein
sequence coverage and identification of post-translational modifications (PTMs), including deamidation and oxidation.

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