High-Speed Process Monitoring with UHP-SEC-MALS

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Size exclusion chromatography (SEC) is widely used for detecting aggregates, fragments, and other impurities in a biotherapeutic sample. When coupled with multi-angle light scattering (MALS), SEC enables researchers to quantify the true solution molar mass of each species in a sample, in addition to the mass fraction of each species. In this application note, we perform rapid UHP-SEC-MALS analysis on two antibodies, mAb1 and mAb2, under various conditions mimicking different manufacturing and purification processing steps. MALS, RI, and dynamic light scattering (DLS) data were collected in each of the 5 minute analyses, providing robust, reproducible, and reliable quantification of molar mass, size, and amount of each species in a given sample.

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