The rise of biotechnology has spawned different means of delivering drugs to the human body. Among the many problems encountered is the rapid rejection or clearance of a protein before it has accomplished its task.
A variety of methods exist for modifying proteins so that they are more effective in the bloodstream. Among them, glycosylation and PEGylation are perhaps the most popular. In the former, sugars are attached to the protein, in the latter, polyethylene glycol strands are attached as “whiskers” to increase the half-life of the protein and make it less likely to be hydrolized or cleared from the bloodstr eam.
In this application note, a monoclonal antibody was measured before and after PEGylation.
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