The use of neonicotinoids, a widely-used class of insecticides, has recently become very controversial as they were pointed as one cause of the honey bees colony collapse disorder. Since pollination is essential for agriculture, extensive studies have been conducted to evaluate the impact of neonicotinoids on bee health, which led to the decision of the European Food Security Authority to strictly limit the use of these pesticides. Consequently, a highly sensitive assay method is now necessary to screen for adherence as well as for delivering a better tool to this emerging research field.
This application news thus describes the ultra-sensitive method for quantitating Acetamiprid, Acetamiprid-N-desmethyl, Clothianidin, Dinotefuran, Fipronil, Fipronil sulfone, Imidacloprid, Nitenpyram, Thiacloprid and Thiamethoxam. LLOQ of single-digit ppt was reached for 6 of the target compounds. Recoveries in honey matrix after QuEChERS extraction was shown to be within the acceptance values 70-120% from EU SANTE/11945/2015 for all compounds. The method was used to quantitate the neonicotinoids in commercially available honey brands far below the authorized maximum residue limit.
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